Helicase Unwinding: Active or Merely Perfect?
نویسندگان
چکیده
منابع مشابه
Mechanisms of helicase-catalyzed DNA unwinding.
DNA helicases are essential motor proteins that function to unwind duplex DNA to yield the transient single-stranded DNA intermediates required for replication, recombination, and repair. These enzymes unwind duplex DNA and translocate along DNA in reactions that are coupled to the binding and hydrolysis of 5'-nucleoside triphosphates (NTP). Although these enzymes are essential for DNA metaboli...
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Helicases are enzymes that use ATP-driven motor force to unwind double-stranded DNA or RNA. Recently, increasing evidence demonstrates that some helicases also possess rewinding activity-in other words, they can anneal two complementary single-stranded nucleic acids. All five members of the human RecQ helicase family, helicase PIF1, mitochondrial helicase TWINKLE, and helicase/nuclease Dna2 hav...
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A prerequisite for DNA replication is the unwinding of duplex DNA catalyzed by a replicative hexameric helicase. Despite a growing body of research, key elements of helicase mechanism remain under substantial debate. In particular, the number of DNA strands encircled by the helicase ring during unwinding and the ring orientation at the replication fork completely contrast in contemporary mechan...
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1 REH2 RNA Helicase in Kinetoplastid Mitochondria: Ribonucleoprotein Complexes and Essential Motifs for Unwinding and Guide RNA Binding Alfredo Hernandez*, Bhaskara Reddy Madina*, Kevin Ro, James A. Wohlschlegel, Belinda Willard, Mike T. Kinter and Jorge Cruz-Reyes Department of Biochemistry and Biophysics, Texas A&M University, 2128 TAMU, College Station, Texas 77843; Department of Biological ...
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Hexameric helicases control both the initiation and the elongation phase of DNA replication. The toroidal structure of these enzymes provides an inherent challenge in the opening and loading onto DNA at origins, as well as the conformational changes required to exclude one strand from the central channel and activate DNA unwinding. Recently, high-resolution structures have not only revealed the...
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ژورنال
عنوان ژورنال: Journal of Molecular Biology
سال: 2012
ISSN: 0022-2836
DOI: 10.1016/j.jmb.2012.04.030